Figure 6.
SOCS3 Overexpression Reverses miR-221 Overexpression-Induced Cell Proliferation, Migration, EMT, Chemotherapy Resistance, and Stem Cell-like Properties in Pancreatic PANC-1 Cells
(A and B) RT-PCR (A) and western blot (B) of SOCS3 after transfection with a SOCS3 overexpression vector or miR-221 mimic alone or combined. Data are means ± SD. ***p < 0.001 versus control. (C) Cell proliferation was examined by CCK8 assays. Data are means ± SD. *p < 0.05 and ***p < 0.001 versus control. (D) Transwell analyses of PANC-1 cell migration (magnification, 200×). (E) The relative migration cells were calculated. Data are means ± SD. ***p < 0.001 versus control. (F) Comparison of cell viability and IC50 of gemcitabine in PANC-1 cells. (G) The relative apoptosis was analyzed. (H) Effect of miR-221 and SOCS3 on gemcitabine-induced apoptosis detected using annexin V-PI double staining after treatment with 10 nM gemcitabine for 72 hr. Data are means ± SD. ***p < 0.001 versus control. (I) E-cadherin, N-cadherin, vimentin, and Snail1 proteins by western blots. (J) Stemness markers OCT4, CD133, Nanog, and SOX2 by western blots.