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P40 peptide vaccine treatment down-regulates colon cytokine production in established TNBS-induced chronic colitis. Frozen colonic samples were mechanically homogenized in buffer supplemented with protease cocktail. Samples were centrifuged to get the supernatants. The concentrations of IL-23, IL-17, TNF, and IL-10 in the supernatants were determined by ELISA. (*, P < 0.05).
