FIGURE 6.

p40 peptide vaccine treatment rebalances Th1/Th17/Treg responses in TNBS-induced chronic colitis. After stimulation with PMA and inomycin, lymphocytes from mesenteric lymph nodes (MLNs) or lamina propria mononuclear cells (LPMCs) were stained with anti-CD4, anti-IFN-γ ,and anti-IL-17 to detect the percentages of CD4⁺IFN-γ⁺Th1 and CD4⁺IL-17⁺Th17 cells by flow cytometry. CD4⁺CD25⁺Foxp3⁺ Treg cells were stained according to the manufacturer’s manual. A, The percentages of Th1, Th17, and Tregs in CD4⁺T cells of MLNs. B, The ratios of Tregs to Th1 and Tregs to Th17 in MLNs. C, The percentages of Th1, Th17, and Tregs in CD4⁺T cells of LPMCs. D, The ratios of Tregs to Th1 and Tregs to Th17 in LPMCs. (*, P < 0.05).