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. 2018 Oct 8;128(11):5073–5082. doi: 10.1172/JCI99933

Figure 6. Mutually exclusive binding of AHSP or eNOS to α-globin.

Figure 6

Myc-tagged eNOS (1 μg) was incubated with purified AHSP (25 ng) and/or α-globin (25 ng) for 90 minutes in TBS at 37°C and then immunoprecipitated with anti-Myc or IgG Dynabeads and analyzed by Western blotting using the indicated antibodies. The lanes were run on the same gel but were noncontiguous. This experiment was conducted 3 times.