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. 2018 Oct 8;128(11):4997–5007. doi: 10.1172/JCI93090

Figure 4. Olanzapine increases plasma MIF levels and MIF expression in adipose tissue.

Figure 4

WT and MIF knockout (Mif–/–) C57BL/6 mice were administered vehicle or 3 mg/kg olanzapine per day for 2 months. Plasma MIF concentrations in WT mice with or without olanzapine treatment were measured by ELISA (A). Adipose, liver, and skeletal muscle tissues were subsequently isolated and (B) tissue MIF content was evaluated by Western blot. Olanzapine stimulation for 24 hours induced a dose-dependent increase in intracellular MIF protein levels in fully differentiated 3T3-L1 adipocytes (C) and induced MIF release into conditioned medium (D) (DMSO was employed as vehicle). Values are mean ± SD. A 2-tailed Student’s t test was used for data analysis in A, B, and D and 1-way ANOVA followed by Tukey’s test was used in C. Mean ± SD; *P < 0.05 versus vehicle or control in A, B, and C, and versus DMSO in D. For each animal group, n = 4–5 in A and B; n = 5 in C, and n = 3 in D.