Figure 1. EMT transcription factors accelerated Kras^G12D-induced lung tumorigenesis.

(A and B) Breeding schematic and bioluminescence imaging and CT scans of CR, CRS, and CRT mice. H, heart; S, spine. (C) Kaplan-Meier curve for tumor-free survival. The number of mice in each group is indicated. ****P < 0.0001, log-rank test. (D) H&E staining of representative mouse lung tumors in B at approximately 40 weeks. Original magnifications: top ×40, bottom ×200, insets ×400. (E–G) IHC of Ki67, p16, and cleaved caspase 3 (CC3) in CR, CRS, and CRT tumors with quantification. Positive staining was quantified in 5 different fields in each section. CR: n = 4, CRS: n = 5, CRT: n = 3. Original magnifications ×100, insets ×200. (H) CT scan, H&E, and SA-βGal staining of representative mouse lung tumor tissues from Kras^G12D (LSL) or Kras^G12D/SNAI1 (CS-LSL) mice. Original magnifications: second column ×4, third column ×100, insets ×200, last column ×200. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, by 1-way ANOVA and Dunnett’s multiple comparisons test in F and G.