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. 2018 Sep 24;128(11):4924–4937. doi: 10.1172/JCI94844

Figure 2. EMT-induced expression of rate-limiting enzymes of the HBP.

Figure 2

(A and B) Enrichment for genes of metabolic process using gene ontology on differentially expressed genes between EMT TF and non–EMT TF–driven mouse tumors. (C–E) qPCR (C) and Western blot (D and E) for the HBP genes Gftp2 and Uap1 in lung tumors (CR: n = 6, CRS: n = 6, CRT: n = 7 mice per genotype). 18s rRNA was used as internal control for all qPCR assays, and CRS and CRT values were normalized to CR. (F) Correlation of EMT TFs SNAI1/TWIST1 and HBP gene GFPT2 in lung adenocarcinoma patient samples from cBIO database. (G and H) Expression of HBP genes in NSCLC cell lines stably infected with SNAI1 or TWIST1. Quantitative PCR was run in triplicate from 2 independent experiments. Unless stated, error bars indicate mean ± SD. P values were derived from 2-way ANOVA and Sidak’s multiple comparisons test in C, G, and H. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.