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. 2018 Sep 24;128(11):4970–4979. doi: 10.1172/JCI99261

Figure 2. Time course of REG3γ and IL-22 expression during GVHD.

Figure 2

(A–C) B6D2F1 mice underwent BMT from syngeneic B6D2F1 donors (no GVHD, white circles) or allogeneic B6 donors (GVHD, black or blue circles), and samples were analyzed on days +3, +7, and +14 after BMT (n = 5 for each group on each day). (A) Serial REG3γ levels in the serum as measured by ELISA. (B) Serial average Paneth cell numbers per HPF in ileal tissue. (C) Serial Reg3g and IL-22 mRNA expression in the ileum was measured by qPCR. (D–G) B6D2F1 mice underwent BMT, without irradiation conditioning, from syngeneic B6D2F1 donors (no GVHD, white triangles) or allogeneic B6 donors (GVHD, black triangles). (D–F) Serial body weight measurement (D), clinical GVHD score (E), and survival (F) of mice after BMT. (G) Serial Reg3g mRNA expression in the ileum on days +3, +7, and +14 after BMT (n = 6 for each group on each day). *P < 0.05, **P < 0.01, unpaired 2-tailed t test (A–E and G); **P < 0.01, log-rank test (F). (H–K) Following irradiation, B6 mice received 5.0 × 106 BM cells plus 0 × 106 to 5.0 × 106 T cells, as indicated, from C3H.SW donors. Samples were analyzed on day +7 after BMT (n = 4 for each group). (H) Body weight measurement. (I) Clinical GVHD score. (J) Serum REG3γ levels. (K) Reg3g mRNA expression in the ileum. *P < 0.05, **P < 0.01, 1-way ANOVA. Data are expressed as mean ± SEM.