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. 2018 Sep 24;128(11):4980–4991. doi: 10.1172/JCI99902

Figure 6. Peli1 expression and its role in human cells during WNV infection.

Figure 6

(A) Immunodetection of Peli1 (green), WNV antigen (WNV Ag) (red), and NeuN (purple), in postmortem hippocampal tissues from 1 control and 2 WNV-infected patients. Nuclei were counterstained with DAPI (blue). Scale bar: 8 μm. Insets are images of sections stained with isotype control antibodies or serum (original magnification, ×63). (B and C) RNA levels of Peli1 in SH-SY5–derived neurons and human microglia on days 1 and 4 p.i. were determined by qPCR. Data are presented as the mean ± SEM and are representative of 2 similar experiments (n = 3–4). ##P < 0.01 compared with the noninfected group (unpaired, 2-tailed Student’s t test). (D–H) SHSY-5Y–derived neurons (D) and human microglial cells (E–H) were treated with control or Peli1 siRNA (Peli1 KD), infected with WNV 385-99 at 48 hours, and harvested at the indicated time points. (D and E) Viral load was measured on day 4 by qPCR. (F–H) IL-6, CCL-2, and CCL-5 production in microglial cells was measured on day 4 by Bio-Plex assay. Data are presented as the mean ± SEM and are representative of 2 similar experiments (n = 4). (D–H) *P < 0.05 and **P < 0.01 compared with the control group (unpaired, 2-tailed Student’s t test).