Fig. 2.
Structural analysis of the ASCCARD filament structure. (A) Surface representation of ASCCARD filament structure, side view and top view. (B) Schematic diagram of the helical filament, with three neighboring subunits highlighted in green, magenta, and cyan. (C) Detailed type I, II, and III interfaces, respectively, of the ASCCARD filament structure. (D) Gel filtration profile of ASCCARD WT and mutants. Void fractions are from elution volumes 7 mL to 9 mL while less aggregated fractions are from elution volumes 14 mL to 17 mL. (E) WT and ASCCARD mutants overexpressed in HeLa cells examined by confocal microscopy. (Scale bar: 10 nm.)