Fig. 3.

Neuroinflammation is not affected by 2DG. (A) Evans Blue stain extraction in sham-infected and PbA-infected mice treated with 2DG or vehicle on day 10 when vehicle-treated mice were exhibiting clinical symptoms with representative photographs (n > 3 per group). Ctl, control. (B) qRT-PCR analyses of IFN-γ (Ifng), TNF-α (Tnf), the type I IFN target gene MX1 (Mx1), and Perforin (Pfn1) on whole brain harvested on day 10 postinfection in vehicle and 2DG-treated mice (n > 4 per group). (C) Flow cytometric quantification of CD8⁺ T cells from whole brain harvested on day 10 (n > 4 per group).