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. 2018 Oct 9;115(43):E10041–E10048. doi: 10.1073/pnas.1810457115

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Fig. 1. — Srs2 cannot remove Dmc1 from ssDNA. (A) Model for mitotic HR highlighting the regulatory role of Srs2 in disruption of Rad51-containing intermediates. (B) Srs2 ATP hydrolysis assays with Rad51 or Dmc1. Data points represent the mean ± SD of three experiments. (C) Schematic of ssDNA curtain assay for Srs2. (D) Kymograph showing unlabeled Srs2 (500 pM) disruption of Rad51-ssDNA as revealed by RPA-GFP (100 pM) rebinding (shown in green). (E) Kymograph showing that unlabeled Srs2 (500 pM) does not dismantle Dmc1-ssDNA.