Fig. 1.

The PUL gene cluster and its phylogenetic distribution. The genome-scale cladogram was constructed using alignments of 1,037 conserved BUSCO genes (22) with newly sequenced genomes in bold. Collapsed clades with multiple (N) species are shown as triangles, and the complete tree, with bootstrap values, is shown in SI Appendix, Fig. S1. Red filled boxes indicate pulcherrimin production has been observed in that species, and filled boxes for PUL1-4 indicate presence or absence of the corresponding PUL gene. Gene arrow diagrams show the arrangement of the four PUL genes in representative species that produce pulcherrimin. The gene table contains putative annotations for the four genes before this study.