Fig. 3.

Mechanism of action of GM12371. (A) Circos plot showing genome-wide differentially expressed lncRNAs and mRNAs from RNAseq analysis (n = 3) of hippocampal neurons transfected with gapmeR B2 to knock down GM12371 and a control nontargeting gapmeR. The outer ring displays the chromosomes annotated with GM12371 and two lncRNA:mRNA pairs. The middle track shows log₂ relative changes of lncRNAs, and the inner track shows log₂ relative change of protein-coding genes belonging to three selected functions (cellular development, growth, and differentiation in red) and all other protein-coding genes (blue). (B) Experimental outline for assessing regulation of lncRNA:mRNA pairs by qPCR. (C–E) qPCR analysis of lncRNAs and mRNAs following GM12371 knockdown n = 5 (C), lncRNA GM10863 knockdown, n = 6 (D), and GM13292 knockdown n = 6 (E). Expression of GAPDH, Kif2A, and CamK2G were used as controls for nonspecific effects of gapmeRs in D and E. Data normalized to 18S rRNA levels. Error bars are SEM, *P < 0.05, **P < 0.01, unpaired two-tailed t test.