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. 2018 Oct 8;115(43):E10197–E10205. doi: 10.1073/pnas.1722587115

Fig. 4.

Fig. 4.

Forskolin regulates the association of GM12371 to transcriptionally active chromatin. (A) Schematics of the native ChIP to assess transcriptionally active chromatin and associated RNAs. A histone marker of transcriptionally active chromatin, H3K27ac, was used in the ChIP analysis. (B) Experimental timeline for GM12371 knockdown and forskolin treatment for ChIP experiments. (C) qPCR analysis of GM12371 and cFOS promoter in ChIP. (D) qPCR analysis of GM12371 and cFOS RNA in ChIP. (E) qPCR analysis of GM12371 and GM10863 lncRNAs in the ChIP following the knockdown of GM12371 by gapmeR B3. A nontargeting gapmeR was used as a specificity control. All groups are normalized to IgG control, before indicated control group normalization. Bar graphs show fold-enrichment in the ChIP following forskolin stimulation. Error bars are SEM, n = 5, unpaired two-tailed t test, *P < 0.05, **P < 0.01. FSK, forskolin; Prm, promoter.