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. 2018 Oct 8;115(43):E10197–E10205. doi: 10.1073/pnas.1722587115

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Copyright © 2018 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 5. — Expression of GM12371 is necessary for the forskolin-induced enhancements in synaptic transmission. (A) Experimental outline for assessing forskolin (FSK)-induced changes in GM12371 and its lncRNA:mRNA targets. (B) qPCR analysis of forskolin-induced changes in the expression of lncRNA:mRNA targets following GM12371 knockdown. Bar graphs show fold-changes. Data normalized to 18s rRNA levels. *P < 0.05, Student’s t test. (C) Experimental outline for assessing the necessity of GM12371 expression for forskolin-induced changes in synaptic transmission. (D) One representative sEPSC traces of control, forskolin, and two representative traces of forskolin + GM12371 knockdown (KD) are shown. (E) Representative sEPSC traces showing changes in amplitude in the presence of forskolin, forskolin + control KD, and forskolin + GM12371 KD. (F and G) Bar graphs showing percent changes in the amplitude and frequency following GM12371 KD in the presence of FSK. ****P < 0.0001, one-way ANOVA followed by Tukey test. (H and I) Cumulative probability analysis showing changes in the amplitudes and frequencies of sEPSCs.