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. 2018 Oct 8;115(43):E10197–E10205. doi: 10.1073/pnas.1722587115

Fig. 6.

Fig. 6.

Regulation of GM10863-Sox10 pair by GM12371. (A) Experimental outline for assessing regulation of GM10863-Sox10 by GM12371. (B) Western blot analysis of Sox10 protein levels following GM12371 knockdown, n = 5, and GM10863 knockdown, n = 3. Sox10 protein levels in protein extracts from hippocampal neurons following lncRNA knockdown using a specific targeting gapmeR and a nontargeting gapmeR are estimated using Western blots. (C and D) Quantification of Western blots in B. Data were normalized to α-tubulin levels. Error bars are SEM, ***P < 0.001, Student’s t test. (E and F) Immunocytochemistry (ICC) analysis of expression of Sox10, a putative cognate mRNA target of lncRNA GM10863 following GM12371 knockdown. (E) Two representative confocal projection images are shown for each condition (gapmeR B3 to knock down GM12371 and control nontargeting gapmeR). (Scale bar, 20 µm.) (F) Quantitation of immunocytochemistry data. Number of neurons analyzed for control gapmeR = 149, gapmeR B3 = 149. CTCF, corrected total cell fluorescence. ***P < 0.001, Student’s t test.