Fig. 5.

Procollagen/LC3 structures rapidly form at COPII puncta, retain COPII coat, and remain relatively stationary. MC3T3 cells transfected with Cherry-Sec23, GFP-proα2^G610C(I), and TagBFP2-LC3 (Left) were imaged by Airyscan (5 s) time-lapse microscopy (Movie S4). Single-slice, time-lapse images (Right) show preexisting long-lived proα2^G610C(I)/LC3/Sec23-positive autophagic structures (white arrows) and formation of a new proα2^G610C(I), LC3, and Sec23-positive autophagic structure (white circle). Blue channels are displayed in cyan for better visualization. [Scale bars: 10 µm (whole cell) and 1 µm (zoom).]