Table 8.
Minimum metrics report based on the submission 123 for the 80S ribosome, including information from the original data collection (Wong et al., 2014).
| Parameter | Example values |
|---|---|
| Data collection | |
| Microscope | FEI Polara |
| Detector | FEI Falcon II |
| Acceleration voltage (kV) | 300 |
| Number of micrographs | 1081 |
| Frames per micrograph | 16 |
| Frame rate (/s) | 16 |
| Dose per frame (e-/pixel) | 2.24 |
| Accumulated dose (e-/Å2) | 20 |
| Defocus range (ʼm) | 0.8 – 3.8 |
| Frames: | |
| Alignment software | motion_corr |
| Frames used in final reconstruction | 1 – 16 |
| Dose weighting | yes |
| CTF: | |
| Fitting software | CTFFIND4 |
| Correction | Full |
| Particles: | |
| Picking software | Relion 1.4 |
| Picked | 123232 |
| Used in final reconstruction | 123232 |
| Alignment: | |
| Alignment software | Relion 1.4 |
| Initial reference map | EMDB 2275 |
| Low-pass filter limit (Å) | 60 |
| Number of iterations | 25 |
| Local frame drift correction | yes |
| Reconstruction: | |
| Reconstruction software | Relion 1.4 |
| Size | 380×380×380 |
| Voxel size (Å) | 1.34 |
| Symmetry | C1 |
| Resolution limit (Å) | 2.68 |
| Resolution estimate (Å, FSC0.143) | 3.1 |
| Masking | no |
| Sharpening | B-factor: −62.4 |