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. 2018 Oct 24;13:6699–6715. doi: 10.2147/IJN.S178809

Figure 5.

Figure 5

Frequency of specific T-helper/memory cells in the blood of vaccinated and virus-challenged pigs.

Notes: PBMCs isolated from pigs at DPC 6 were analyzed for the frequency of T-helper/memory cells (CD3+ CD4+ CD8α+) upon re-stimulation with: (A) ten pooled peptides; (B) H1N1-OH7; (C) peptide 1; (D) peptide 2; (E) peptide 3; (F) peptide 4; (G) peptide 5; (H) peptide 6; (I) peptide 7; (J) peptide 8; (K) peptide 9; and (L) peptide 10. Each bar represents the mean ± SEM of 4–6 pigs. Data were analyzed by non-parametric Kruskal-Wallis test followed by Dunn’s post hoc test. Asterisk refers to statistical difference between two indicated pig groups (*P<0.05, **P<0.01, and ***P<0.001).

Abbreviations: DPC, day post-challenge; NPs, nanoparticles; PBMCs, peripheral blood mononuclear cells; SEM, standard error of mean.