Figure 7.

Secretion of Th1 cytokine IFN-γ by PBMCs of liposome peptide cocktail vaccinated and virus-challenged pigs.

Notes: PBMCs isolated from pigs at DPC 6 were re-stimulated with (A) pooled peptides; (B) H1N1-OH7; (C) peptide 1; (D) peptide 2; (E) peptide 3; (F) peptide 4; (G) peptide 5; (H) peptide 6; (I) peptide 7; (J) peptide 8; (K) peptide 9; and (L) peptide 10, and the harvested culture supernatants were analyzed for IFN-γ by ELISA. Each bar represents the mean ± SEM of 4–6 pigs. Data were analyzed by non-parametric Kruskal-Wallis test followed by Dunn’s post hoc test. Asterisk refers to statistical difference between two indicated pig groups (*P<0.05).

Abbreviations: DPC, day post-challenge; NPs, nanoparticles; PBMCs, peripheral blood mononuclear cells; SEM, standard error of mean.