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. 2018 Oct 29;13(10):e0205518. doi: 10.1371/journal.pone.0205518

Fig 5. Differential expression pattern of NF-κB family proteins in OSCC cell lines.

Fig 5

A. Western blot analysis of NF-κB family proteins in cellular protein extracted from sorted SP, NSP and parental cells from (i)UD-SCC2 HPV16+, (ii) UPCI:SCC131 and (iii) UPCI:SCC84 cell line. A total of 25 μg protein extracts each from SP, NSP and parental cells were separated on a 10% SDS-PAGE, electrotransferred on PVDF membrane and probed for NF-κB family proteins p65, p50, p52, c-Rel and Rel-B. To confirm equal protein loading, the membranes were reprobed for β-actin expression. The relative normalized fold change in the protein is expressed as the mean ±SD of three independent experiments. B. Bar diagram showing NF-κB family proteins expression between SP cells, NSP cells and Parental cells from (i) UD-SCC2 HPV16+, (ii) UPCI:SCC131 and (iii) UPCI:SCC84 cell lines. ***P value <0.001, **P value <0.01, *P value <0.05.