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. Author manuscript; available in PMC: 2018 Oct 29.
Published in final edited form as: J Nucl Med. 2014 Mar 6;55(4):686–694. doi: 10.2967/jnumed.113.127480

Figure 1. Generation of highly invasive mammary carcinoma cell lines stably expressing NIS-TagRFP and CXCR4-GFP variants.

Figure 1

(A) Retroviral constructs for human NIS-TagRFP (left) and wildtype (FL) or truncated (Δ) CXCR4-GFP (right). (B) NIS-TagRFP function was measured by 99mTcO4- uptake and compared to cell lines not expressing NIS-TagRFP. Perchlorate inhibition was used to demonstrate uptake specificity. Error bars represent SD; N=3. (C) Representative confocal micrographs of double-stable cell lines showing expected plasma membrane localization of both fusion proteins.