Figure 4. ATP binding, but not hydrolysis, is required for cohesin loading.
(A) Gel image and quantification of recovered DNA from cohesin loading reactions performed with or without added ATP. (B) Gel image and quantification of recovered DNA from cohesin-loading reactions performed with wild-type or Walker B motif mutant EQ-cohesin. (C) An assay in which ATP and the indicated nucleotide derivatives were compared for their ability to support cohesin loading. The mean values and standard deviations from three independent experiments are shown in panels (A–C). (D) The topological nature of cohesin loading, supported by ADP·AlFx, was analyzed following DNA linearization. (E) Fission yeast cohesin loading onto DNA was measured in the presence of ATP or the indicated nucleotide derivatives.