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. 2018 Oct 26;1(5):e201800143. doi: 10.26508/lsa.201800143

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© 2018 Minamino et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure S4. — (A) Gel image of input DNA of various topologies and of recovered DNA following cohesin loading. (B) Topological DNA loading promoted by Scc2C was assessed by DNA release following linearization with PstI. Supernatant (S) and bead-bound fractions (B) were retrieved following PstI or mock treatment. DNA in both fractions was analyzed by agarose gel electrophoresis. CCC, covalently closed circular DNA; L, linear DNA; NC, nicked circular DNA; RC, relaxed circular DNA.