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Optimisation of His-tag sequences for 99mTc tricarbonyl labelling from high throughput screening. Top: relative labelling efficiency of a range of peptides. Purple symbols represent His-tags with adjacent arginine or lysine residues (i.e. positively charged sequences). Other colours represent uncharged and negatively charged sequences. Bottom: Comparison of labelling efficiency of optimal binding sequence selected from the screening (green)53 with established his tags sequences.48–50
