Fig 6. In vitro enzymatic activities of HCTRs encoded by the new APR alleles of hm1.
Protein extracts from the leaf tissue of near-isogenic lines of the APR alleles Hm1-3 and Hm1-4 in the B73 background were used to conduct in vitro HCTR assays. The fully resistant (Hm1B73) and susceptible (hm1-2) alleles of hm1 were used as controls. HCTR activities, measured at age week-3 and week-7, relied on determining the amount of HC-toxin reduced via LC-MS/MS. Letters represent whether differences among each age group were significant (padj < 0.05).