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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Autoimmun. 2018 Feb 1;89:139–148. doi: 10.1016/j.jaut.2017.12.013

Figure 1. Treg-specific deletion of NKAP recapitulates the “scurfy” phenotype by the age of three weeks.

Figure 1

(A) Foxp3-YFP-cre NKAP cKO male mice juxtaposed with wildtype (WT) and Foxp3 mutant scurfy mice as well as spleens, lymph nodes (LN) and thymi. (B) Examination of absolute cell counts of splenocytes, lymph node cells and thymocytes. Bar graphs are averaged absolute cell counts per organ from at least 5 independent experiments with at least 5 mice per group, and error bars are standard error of mean (SEM). (C) Hematoxylin and Eosin staining from sections from Foxp3-YFP-cre NKAP cKO male mice and WT littermate lungs, liver and kidneys at three weeks of age. Data is representative of three organs per genotype. (D) Presence of antinuclear antibodies (ANA) in WT or cKO serum was determined by incubating serum with irradiated HepG2 cells, followed by detection using fluorescent anti-mouse secondary antibody. Data is representative of 15 WT and 17 Foxp3-YFP-cre NKAP cKO male mice. (E) Detection of anti double stranded DNA (dsDNA) antibodies. Data is averaged from of 15 WT and 17 Foxp3-YFP-cre NKAP cKO male mice.