Fig. 5.

Newly discovered, hotspot-enriched proteins are bona fide regulators of hotspot activation. a The frequencies of meiotic recombination were determined in test crosses harboring hotspot (M26) and basal control (M375) alleles of ade6 in the chromosome. b Example of a hotspot-specific regulator, plotted using recombinant frequencies. Note that the removal of the Hip1 (Hir1) protein reduces recombination at M26 (hotspot) but not at M375 (basal recombination control), indicating that the basal recombination machinery is intact. c The frequencies of hotspot recombination at M26 were determined in strains with the indicated genotypes and are plotted as percent activity relative to that in wild-type cells. d As in “c,” but showing the effects of the null mutations on basal recombination at M375. Data are mean ± SD from three or more biological replicates