Table 2. Antibodies used for immunohistochemistry.
| Antigen | Dilution | Supplier | Catalogue no. |
|---|---|---|---|
| Laminin |
1:200 |
Sigma (Poole, UK) |
#L9393 |
| Fibronectin |
1:200 |
Sigma (Poole, UK) |
#F3648 |
| αSmooth Muscle Actin (αSMA) |
1:100 |
Sigma (Poole, UK) |
#A5228 |
| Phosphorylated Myosin Light Chain (MLCp) |
1:400 |
Abcam (Cambridge, UK) |
#ab2480 |
| RhoA |
1:200 |
Abcam (Cambridge, UK) |
#ab54835 |
| Brn3a |
1:100 |
Santa Cruz (Santa Cruz, CA) |
#SC-31984 |
| Mouse IgG (Fluor 488) |
1:400 |
Molecular Probes (Paisley, UK) |
#A-21202 |
| Rabbit IgG (Fluor 488) |
1:400 |
Molecular Probes (Paisley, UK) |
#A-21206 |
| Rabbit IgG (Fluor 594) |
1:400 |
Molecular Probes (Paisley, UK) |
#A-21207 |
| Goat IgG (Fluor 594) | 1:400 | Molecular Probes (Paisley, UK) | #A-11058 |