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. 2018 Oct 29;7:e37549. doi: 10.7554/eLife.37549

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© 2018, Knight et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) Schematic of micropatterned tissue derivation for D^-1 hESCs and D4 NECs; sub-culture/seeding onto micropatterned substrates indicated by (*). (B) Representative immunostained images of forebrain, micropatterned neuropithelial tissues. (C) Manual quantification of polarization foci/neural rosettes per tissue with the number of tissues (technical replicates) analyzed per condition indicated above each bar. (D) Average cell density and (E) area of polarized N-cadherin ring within micropatterned neuroepithelial tissues derived from D^-1 hESCs and D4 NECs. Number of tissues analyzed per condition indicated on each bar, and error bars represent standard deviation. (*) indicates a significance of p<0.05 calculated using a One-way ANOVA. (F) Representative confocal Z-stack images of micropatterned neuroepithelial tissues (top) with profile view of red highlighted region (bottom). Scale bars are (B, F-top) 100 μm and (F-bottom) 10 μm.

Figure 3— source data 1. Quantification of rosette emergence, tissue cell density, and polarized N-cadherin ring area.

elife-37549-fig3-data1.xlsx^{(50KB, xlsx)}

DOI: 10.7554/eLife.37549.009