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. Author manuscript; available in PMC: 2018 Oct 30.
Published in final edited form as: Cell Stem Cell. 2017 Oct 5;21(4):431–447. doi: 10.1016/j.stem.2017.09.006

Figure 1. CRISPR/Cas9-based applications to study and manipulate the epigenome.

Figure 1.

Main applications of the CRISPR/Cas9 system to study and manipulate the epigenome are based on the feasibility to allocate chromatin modifiers and fluorescent molecules in a very precise way. This includes the targeted reposition of transcriptional regulators, histone modifiers, enzymes responsible for changes in the DNA methylation, chromatin-interacting ncRNAs, and fluorescent molecules. (Grey dotted-boxes can be replaced with the colored-boxes corresponding to reported examples of chromatin-modifiers and fluorescent molecules). HDM: histone demethylase, HMT: histone methyltransferase, HAT: histone acetyltransferase, HDAC: histone deacyetylase, HUbq: histone ubiquitin ligase, DNMT: DNA methyltransferase, TET: ten-eleven translocation enzymes. Ac: acetylation, Me: methylation, Ub: ubiquitination.