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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Curr Protoc Protein Sci. 2018 Aug 16;94(1):e65. doi: 10.1002/cpps.65

Figure 4.

Figure 4

Extraction of peptides from an entire tissue slice for identification by LC-MS/MS proteomics. Solvent is placed on the tissue forming a bead and allowed to incubate for 3 min, covered. A pipette tip is inserted at a 45° angle on the edge of the tissue and bead of solvent. The solvent is aspirated into the pipette tip and placed in a microcentrifuge tube.