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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: FEBS Lett. 2018 Sep 24;592(20):3429–3437. doi: 10.1002/1873-3468.13249

Figure 2. RALF1-mediated regulation of stomatal movement is not restored in fer-4 pFER::FERK565R-GFP plants with fer-like morphology, but is restored in pFER::FERK565R-GFP plants with wild-type morphology, corresponding to FERK565R-GFP transgene overexpression.

Figure 2.

(A) RALF1 inhibition of stomatal opening. Leaf pieces were incubated in the dark to close the stomata for 2 h and then treated with or without 10 μM RALF1 in the light for another 3 h. Plants used in this assay are the same plants as were used for transcript quantification in Fig. S1A. (B) RALF1 promotion of stomatal closure. Leaf pieces were incubated in the light for 3 h to open the stomata and then treated with or without 10 μM RALF1 in the light for another 2 h. The results are mean ± S.E. for 3 independent experiments with over 80 apertures measured per genotype per treatment. Significant differences between control and treatment (Student’s t-test) are indicated (*P < 0.05).