Table 1.

UHPLC-MS/MS parameters.

UHPLC Conditions
Column	Kinetex® C18, 100 mm × 2.1 mm, 1.7µm particle diameter
Injection volume	10 µL
Flow rate	0.4 mL/min
Mobile phase	90% methanol with 0.1% formic acid
MS/MS Conditions
Declustering potential	80 V
Entrance potential	10 V
Collision energy	25 V
Collision cell exit potential	20 V
APCI Conditions
Nebulizer current	3 mA
Temperature	300°C
Curtain gas	20 psi
Ion source gas	55 psi