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. Author manuscript; available in PMC: 2019 Oct 30.
Published in final edited form as: Circulation. 2018 Oct 30;138(18):1988–2002. doi: 10.1161/CIRCULATIONAHA.118.034189

Figure 1. A2R mediates the protective effect of PDE1C inhibition or deficiency on Ang II-induced cardiomyocyte death.

Figure 1

(A–C) Cardiomyocytes isolated from PDE1C-WT or -KO mice were stimulated with 200 nmol/L Ang II for 24 hours in the presence of IC86340 (15 μmol/L), A2R antagonist (A2R-I, ZM241385, 200 nmol/L) or vehicle; (A) Trypan blue staining. White arrows indicate trypan blue-positive myocytes. The scale bar is 50 μm. (B–C) Quantification of trypan blue staining results. 20 random fields and approximate average of 300 myocytes were counted per dish. Data were presented as mean ± SEM. *p<0.05, ns: not significant by one-way ANOVA with Bonferroni’s correction. B: n=6 replicates from 3 mice. C: n=5 replicates from 3 mice.