Figure 13. Phase diagrams of αSN aggregation on model membranes in initial states.

(A–D) The phase states of αSN_WT (A and C) and αSN₁₀₃ (B and D) in the presence of DOPC (A and B) and Mimic SUV (C and D) in the initial states before the incubation. Regions of unstructured and helically-folded αSN are shown in blue and light green, respectively. Denser colors represent greater possibilities for the existence of corresponding molecular species depending on the concentrations of αSN and lipids. The yellow broken lines of the conceptual solubility curves in each phase diagram are represented for comparing solubility before (Figure 13) and after equilibrium (Figure 14) (i.e., before and after the disruption of the metastability of supersaturation). Broken black lines are also shown to represent the conceptual concentration of αSNs used in the present study for a better understanding of molecular species. Molecular species that are present under each condition are schematically shown. Three major regions of αSN are represented using different colors, as described in Figure 10A. Increases in the content of α-helix in αSNs with higher concentrations of lipids are shown above individual phase diagrams. RCS, PHS, and LHS indicate random coil-like structures with less helicity, partially-helical structures, and largely-helical structures, respectively. X and Y axes indicate the concentrations of lipids and αSNs, respectively. This figure is reproduced from reference [149].