Fig. 2. VPA reduced the levels of methyl-CpG-binding protein 2 (MeCP2) expression in NPCs.

Cultured NPCs were treated with 0.2 or 0.5 mM VPA for 3 h. (A, B) MeCP2 mRNA levels were analyzed with RT-PCR (A), and protein levels were analyzed with western blots (B). All data are expressed as mean±SEM (n=3). ^**p<0.01 and ^***p<0.001 vs. Vehicle 3 h (one-way ANOVA followed by post hoc Tukey's comparisons test). (C) Immunostaining of MeCP2 and diamidino-2-phenylindole (DAPI) in VPA-treated NPCs. The arrow indicates the coexpression of MeCP2 and DAPI, while the arrowhead indicates the region in which MeCP2 was expressed but DAPI staining cannot be observed. The magnified images are from the top row. All data are expressed as mean±SEM (n=3). ^**p<0.01 and ^***p<0.001 vs. Vehicle 3 h (one-way ANOVA followed by post hoc Tukey's comparisons test). Scale bar=10 µm.