Fig. 4. Effects of C3G on Aβ25–35-induced mitochondrial depolarization.
(A) Reproducible depolarization in mitochondrial membrane potential (MMP) was elicited by applying Aβ25–35 (20 µM) for 5 min at 35 min intervals. (B) Pretreatment with C3G (10 µg/ml) for 30 min significantly inhibited Aβ25–35-induced mitochondrial depolarization. (C) Summary of glutamate-induced mitochondrial depolarizations in non-treated (control, n=18) and C3G-pretreated (n=18) cells. Cells were preincubated with 10 µM rhodamine 123 for 15 min. Change in MMP was shown as a percentage of the maximal intensity of rhodamine 123 (3 µM FCCP). Data are expressed as mean±S.E. **p<0.01 relative to control (non-paired Student's t-test).