Table 1.
Structural and biological properties of KP32gp37 and KP32gp38.
| Properties | KP32gp37 | KP32gp38 | Method used |
|---|---|---|---|
| Capsular-type specificity | K3 | K21 | Spot test |
| Number of amino acids | 869 | 578 | ExPASy - ProtParam tool |
| Theoretical pI | 5.28 | 7.57 | ExPASy - ProtParam tool |
| Predicted molecular mass (kDa) | 95.35 | 61.87 | ExPASy - ProtParam tool |
| Oligomeric state (kDa) | 259.5 ± 0.52 | 179.4 ± 0.54 | Size-exclusion chromatography coupled with light scattering |
| Secondary structure | Mainly β-sheet | Mainly β-sheet | CD spectroscopy |
| Melting temperature (Tm) | 74°C | 56°C | CD spectroscopy |
| Thermostability | Up to 45°C | Up to 50°C | ONPG assay |
| pH stability | 4–9 | 6–9 | ONPG assay |
| Susceptible to SDS | Resistant | Sensitive | Electrophoresis |
| Susceptible to proteolysis | Resistant | Sensitive | Electrophoresis |