FIGURE 7.

In mycelia the MAPKK PbsB is necessary for MpkC nuclear accumulation in response to oxidative and osmotic stress. (A) Mycelia from strain TRJ13 (MpkC::GFP H2A::mRFP) grown for 14 h in minimal medium (Top) was treated or not with 10 mM H₂O₂ for 20 min (Middle) or grown for 14 h on MM containing 1.2 M sorbitol and then observed using confocal microscopy. (B) Mycelia from strain CVG10 (ΔpbsB MpkC::GFP H2A::mRFP) grown for 14 h in minimal medium (Top) was treated or not with 10 mM H₂O₂ for 20 min (Middle) or grown for 14 h on MM containing 1.2 M sorbitol and then observed using confocal microscopy. White arrowheads in panel (A) point to some of the nuclei with MpkC::GFP signal.