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. 2018 Oct 23;9:2524. doi: 10.3389/fmicb.2018.02524

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Copyright © 2018 Al-Bassam, Haist, Neumann, Lindenberg and Tschowri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression profiles of c-di-GMP metabolism genes and proteins during submerged sporulation of S. venezuelae. (A) Expression data were extracted from microarray analyses in Bibb et al. (2012) and normalized with quantile normalization and median polish using the RMA method. Expression values are shown as Log2. (B) Western blot analysis of FLAG-tagged proteins. GGDEF/EAL/HD-GYP-domain proteins were FLAG-tagged and expressed in the corresponding mutants. Protein samples were harvested after indicated time of growth and following amounts of total protein were used for Western blotting: 5 μg for CdgB-FLAG; 10 μg for CdgE-FLAG, RmdB-FLAG and HdgB-FLAG; 15 μg for CdgA-FLAG, CdgC-FLAG, and RmdA-FLAG; 20 μg for CdgD-FLAG, CdgF-FLAG, HdgA-FLAG. co, wildtype sample as control.