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. 2018 Oct 23;9:2524. doi: 10.3389/fmicb.2018.02524

FIGURE 3.

FIGURE 3

CdgC is an active DGC. (A) Synthesis of c-di-GMP from GTP was assayed in vitro using purified cytosolic fraction of CdgC (ΔTM-CdgC; TM: transmembrane) and PleD and visualized by thin layer chromatography. (B) The cdgC mutant and a strain carrying a chromosomal cdgC version with the ALLEF motif in the active site show green wildtype morphology when complemented with cdgC from the pMS82 vector but remain gray and do not form aerial mycelium with the empty vector. Overexpression of CdgB can partially suppress the cdgC deletion. Strains were grown for 3 days on MYM agar.