Figure 2.

Intravitreal AAV injection procedure. (A) Depiction of the mouse eye (frontal view) with a dilated pupil held by the scleral connective tissue with forceps. Using a sharp 30G needle (e.g., insulin syringe) an incision is made into the sclera. (B) A Hamilton syringe with a 34G blunt needle is carefully inserted into the incision and the AAV viral suspension is slowly injected into the vitreous. By adding fluorescein dye (green) to the viral suspension, the intravitreal injection can be observed by the homogenous diffusion of the green dye inside the vitreous.