Figure 6.

Abundant IL-6 and IL-10 release by MDSCs upon Mtb infection are regulated by NF-κB and MAPK pathways. IL-6 and IL-10 were measured in cell-free culture supernatants from Mtb-infected cells (MOI 10) by ELISA (A–C). Cells were treated with indicated inhibitors and supernatants were collected at 24 h post infection. Each symbol corresponds to a single donor, shown are median ± IQR. Data are pooled from 3 independent experiments (n = 6–8 individuals); one-way ANOVA with Dunn's multiple correction or Wilcoxon test (if only 2 conditions were available), ^*p ≤ 0.05 and ^**p ≤ 0.01, ^***p ≤ 0.001.