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. 2018 Oct 23;9:2417. doi: 10.3389/fimmu.2018.02417

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Copyright © 2018 Agrawal, Streata, Pei, Weiner, Kotze, Bandermann, Lozza, Walzl, du Plessis, Ioana, Kaufmann and Dorhoi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interference with MAPK restricts bacterial replication in MDSCs co-cultured IVGLSs. Selected concentrations of inhibitors were added to co-cultures of IVGLSs and MDSCs/MDMs at the time of addition of the myeloid cells, that is day 5 post IVGLSs generation. CFUs were estimated after 48 h of co-culturing. Each symbol corresponds to a single donor, shown are median±IQR. Data are pooled from 2 independent experiment (n = 6 individuals); one-way ANOVA with Dunn's multiple correction [for conditions of (i) IVGLSs, (ii) MDSC co-cultures, and (iii) MDM co-cultures], ^***p ≤ 0.001, ^****p ≤ 0.0001. Paired Student's t-test was performed only for untreated conditions.