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. 2018 Oct 29;8:15983. doi: 10.1038/s41598-018-34258-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of V158F polymorphism by showing 3 different genotypes, homozygous T, homozygous G, and heterozygous. (a) Electropherograms show the Sanger-based sequencing result around the V158F polymorphism. The red square indicates nucleotide position 5064, which is used to check whether the FCGR3B gene is co-amplified. The yellow square indicates nucleotide position 5093, used for determining the V158F polymorphism. Nucleotide code K indicates that both T and G are present. (b) MinION sequencing result around the V158F polymorphism. Dark grey bars on the top show the sequence coverage identical to the consensus sequence. If the sequence is not identical to the consensus the bars will have the color of the corresponding nucleotide. The light grey lines show a small part of the reads obtained with the MinION run and the sequence at the bottom shows the consensus sequence. The first result represents a sample homozygous for T at position nucleotide 5093 (coverage: A: 0%: C: 2% G: 2% T: 96%), which was also used as consensus, the second sample is homozygous for G (coverage: A: 3% C: 2% G: 85% T: 9%), and the third is heterozygous at nucleotide position 5093 (coverage: A: 1% C: 3% G: 32% T: 64%).