Fig. 3. CFO maintains self-renewal of human HSPCs.

a Schematic diagram of molecular regulators in HSPCs. Factors (CHIR-99021, Forskolin and OAC1) that have a demonstrated effect on human HSPCs are shown, including transcription factors and signaling pathways targeted for stem cell maintenance. b Changes in expression levels of CHIR-99021, Forskolin and OAC1 targets measured by qRT-PCR after a 7-day culture with CFO compared with control. Data represents the mean ± SD. c Gene expression profile of CFO. CFO group was randomly picked to perform single-cell qRT-PCR assay with selected genes from early human haematopoietic lineages. C, CHIR-99021; F, Forskolin; O, OAC1