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. 2018 Oct 30;4:59. doi: 10.1038/s41421-018-0059-5

Fig. 4. Combination of CFO and HGF increases functional human HSPCs.

Fig. 4

a Relative expression of CD34. Human CD34-positive cells exposed to C, F, O, CF, CO, FO and CFO on the basic medium with haematopoietic growth factor (HGF) were assayed for relative expression of CD34. *P < 0.05. Data represents the mean ± SD. b Clone numbers. Human CD34-positive cells exposed to CFO, HGF and CFO + HGF were assayed for colony-forming units. G, granulocyte; E, erythroid; M, myeloid; GM, granulocyte/monocyte; GEMM, granulocyte, erythroid, monocyte and megakaryocyte colonies. *P < 0.05. Data represents the mean ± SD. c Phenotype of HSC. Uncultured human CD34-positive cells and these cells exposed to CFO, HGF and CFO + HGF were assayed for CD34, CD38, CD45RA, CD90 and CD49f by flow cytometry. C, CHIR-99021; F, Forskolin; O, OAC1; HGF, stem cell factor (SCF) and thrombopoietin (TPO)