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. 2018 Oct 30;4:59. doi: 10.1038/s41421-018-0059-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a T-Distributed Stochastic Neighbor Embedding (t-SNE) analysis identified 11, 3, 4, 8 and 8 subpopulations in 5 samples: uncultured human CD34-positive cells (J1), control cells (J2), cells with the treatment of CFO (J3), HGF (J4) and CFO + HGF (J5). b Dot plot visualization of each subpopulation in the above 5 samples. The size of the dot encodes the percentage of cells within a subpopulation, while the color encodes the average expression level of ‘expressing’ cells. c Expression of selected genes across every subpopulation in the above 5 samples. 100 cells from each subpopulation were randomly sampled three times for gene expression analysis. C, CHIR-99021; F, Forskolin; O, OAC1; HGF, stem cell factor (SCF) and thrombopoietin (TPO)